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There are alot more pics but it takes a rather long time to upload so I picked out the beautiful ones :)
Starting with transferring the blood to another tube and washing it with tris-EDTA.
To incubating them on ice.
You can see the pellet containing the DNA after blood has been washed out.
A side view of the pellet.
Adding phenol chloroform to further isolate the DNA.
Shaking the contents with phenol chloroform turns it milky.
After centrifugation you can see the two layers of where the lower layer will be removed.
Removing the lower layer with a syringe to get as much of the contents of the layer out.
After precipitation with sodium chloride and 100% ethanol you can see the DNA floating in the solution.
And after the long steps of drying and solubilising, the DNA is ready to be quantitated using a quartz cuvette in the spectrophotometer.
8 comments:
and this little Miss Photographer happens to be me lor...hehehe...next time pose more ya then can become model de...only your hands la...kekeke :p
-Joyce-
ermm u making test tube babies ah? kakaka... takes 10 mths to make wor. boy or girl ah? or gay? hehehe
Oi, achei teu blog pelo google tá bem interessante gostei desse post. Quando der dá uma passada pelo meu blog, é sobre camisetas personalizadas, mostra passo a passo como criar uma camiseta personalizada bem maneira. Até mais.
More body pics pls.. :P
That was me. :P Stupid internet.
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